human gc cell line ags  (Procell Inc)

Journal: Oncology Reports

Article Title: NR4A1 mediates chemotherapy-induced senescence via the PI3K/AKT pathway in gastric cancer cells

doi: 10.3892/or.2026.9080

Figure Lengend Snippet: OXA induces senescence was verified in gastric cancer cells. (A and B) SA-β-Gal staining of Ctrl and OXA groups and quantification of SA-β-Gal positive cells in AGS and MKN45 cells. (C) p21 protein expression and quantification. (D and E) Immunofluorescence of γ-H2AX and quantification. Red fluorescence indicates γ-H2AX staining, and blue fluorescence reflects nuclear staining with DAPI. (F) mRNA levels of the senescence-associated secretory phenotype factors. (G) CDK4, cyclin D1, RB, p-RB protein expression, and quantification in the two groups. Data represent the mean ± SD of at least three independent experiments. *P<0.05, **P<0.01 and ***P<0.001. OXA, oxaliplatin; SA-β-Gal, senescence-associated β-galactosidase; p-, phosphorylated.

Article Snippet: AGS and MKN45 human GC cell lines were acquired from the Procell Life Science & Technology Co., Ltd. and maintained in RPMI-1640 containing 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) at 37°C with 5% CO 2 condition.

Techniques: Staining, Expressing, Immunofluorescence, Fluorescence

Journal: eLife

Article Title: Blocking SHP2 benefits FGFR2 inhibitor and overcomes its resistance in FGFR2 -amplified gastric cancer

doi: 10.7554/eLife.104060

Figure Lengend Snippet: Sensitivity of KATOIII and SNU-16 to ( A ) SHP099, ( B ) AZD4547, or combination therapy with different concentration gradients (n=4; two-way ANOVA). Effects of different treatments on cell apoptosis of ( C ) KATOIII and ( D ) SNU-16 after 48-hour drugs incubation (n=3; one-way ANOVA). ( E ) KATOIII and ( F ) SNU-16 were incubated with vehicle, SHP099 10 μM, AZD4547 3 nM or combination therapies for 1 hour or 48 hours, then cell lysates were immunoblotted for phospho-FGFR and total-FGFR2, phospho-SHP2 and total-SHP2, phospho-Erk and total-Erk, phospho-p38 and total-p38, phospho-AKT and total-AKT, and phospho-mTOR and total-mTOR. Data are shown as mean ± SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. p-Values are determined by ordinary one-way ANOVA or two-way ANOVA. Figure 2—source data 1. PDF file containing original western blots for , indicating the relevant bands. Figure 2—source data 2. Original file for western blots displayed in .

Article Snippet: Human GC cell lines SNU-16 (ATCC CRL-5974), MKN45 (KANGBAI CBP60488), NUGC4 (KANGBAI CBP60493), HGC27 (KANGBAI CBP60480), and SNU601 (KANGBAI CBP60507) were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China).

Techniques: Concentration Assay, Incubation, Western Blot

Journal: eLife

Article Title: Blocking SHP2 benefits FGFR2 inhibitor and overcomes its resistance in FGFR2 -amplified gastric cancer

doi: 10.7554/eLife.104060

Figure Lengend Snippet: BALB/c nude mice were injected with 1×10 7 SNU-16 cancer cells and received different formulations by oral gavage every day upon tumor volumes reached 100–150 mm 3 . ( A ) Schematic of SHP099 and AZD4547 therapeutic schedule in FGFR2 -amplified SNU-16 subcutaneous xenograft model. ( B ) Representative images of tumors (n=5). ( C ) Tumor volumes (n=5; two-way ANOVA), ( D ) tumor weights (n=5; one-way ANOVA) and ( E ) body weights (n=5; two-way ANOVA) of different groups. ( F ) Tumors were harvested 6 hours after the last dose of drugs, and cell lysates from tumor tissues were immunoblotted for phospho-FGFR and total-FGFR2, phospho-SHP2 and total-SHP2, phospho-Erk and total-Erk, phospho-AKT and total-AKT, and phospho-mTOR and total-mTOR. Data are shown as mean ± SEM. ns, not significant, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. p-Values are determined by ordinary one-way ANOVA or two-way ANOVA. Figure 4—source data 1. PDF file containing original western blots for , indicating the relevant bands. Figure 4—source data 2. Original file for western blots displayed in .

Article Snippet: Human GC cell lines SNU-16 (ATCC CRL-5974), MKN45 (KANGBAI CBP60488), NUGC4 (KANGBAI CBP60493), HGC27 (KANGBAI CBP60480), and SNU601 (KANGBAI CBP60507) were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China).

Techniques: Injection, Amplification, Western Blot

Journal: eLife

Article Title: Blocking SHP2 benefits FGFR2 inhibitor and overcomes its resistance in FGFR2 -amplified gastric cancer

doi: 10.7554/eLife.104060

Figure Lengend Snippet: ( A ) Overview of FGFR2 alterations individually in GC patients from Nanjing Drum Tower hospital cohort. ( B ) PD-L1 mRNA expression levels were analyzed between FGFR2 -amplified group (n=13) and FGFR2 -unamplified group (n=250) from TCGA-STAD cohort (Welch’s t -test). ( C ) Proportions of MSI-H or MSS in FGFR2 -amplified group (n=12) and FGFR2 -unamplified group (n=237) from TCGA-STAD cohort (Fisher’s exact test). Human peripheral blood mononuclear cells (PBMCs) were incubated with different administrations in the presence of 0.25 μg/ml human anti-CD3 and 1 μg/ml human anti-CD28. Proportions of ( D ) IFN-γ/CD8+ cells were detected by flow cytometry assay after 24 hours of drugs incubation (n=3; one-way ANOVA). ( E ) Expression levels of IFN-γ in cellular supernatant were measured by Cytometric Bead Array (CBA) assay after 24 hours of drugs incubation (n=3; one-way ANOVA). ( F ) Cell viability of human PBMCs after 48 hours of drugs incubation (n=4; one-way ANOVA). ( G, H ) After 48 hours of advance drugs stimulation in human PBMCs, cytotoxicities of human PBMCs against SNU-16 at different E:T ratios of 10:1, 20:1, 40:1 were analyzed by flow cytometry assay after CFSE/PI staining (n=3; two-way ANOVA). Data are shown as mean ± SEM. ns, not significant, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. p-Values are determined by Welch’s t -test, Fisher’s exact test, ordinary one-way ANOVA or two-way ANOVA.

Article Snippet: Human GC cell lines SNU-16 (ATCC CRL-5974), MKN45 (KANGBAI CBP60488), NUGC4 (KANGBAI CBP60493), HGC27 (KANGBAI CBP60480), and SNU601 (KANGBAI CBP60507) were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China).

Techniques: Expressing, Amplification, Incubation, Flow Cytometry, Staining